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goat polyclonal antibody against mouse il 36α  (R&D Systems)


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    R&D Systems goat polyclonal antibody against mouse il 36α
    Total RNA and formalin-fixed paraffin sections were prepared from the kidneys from mice used in Fig. . Relative mRNA levels of renal tubular damage markers were measured by quantitative RT-PCR. a Osteopontin. b Neutrophil gelatinase-associated lipocalin (Ngal). c Kidney injury molecule-1 (Kim-1). d <t>Interleukin-36α</t> (IL-36α). e Interleukin-6 (IL-6). Data are indicated as violin plots with the median and quartiles (dotted lines). N = 6 for each column. * P < 0.05, # P < 0.01 versus baseline by one-way ANOVA with Tukey’s multiple comparison test. f Plasma creatinine levels. Data are presented as means ± SD. N = 6 for each column. No statistical differences were observed between the groups by one-way ANOVA with Tukey’s multiple comparison test. g Representative kidney section images of immunohistochemistry using antibodies against osteopontin (upper panels) or IL-36α (lower panels) at the indicated time points after the last sRANKL or vehicle injection. Bar = 1 mm.
    Goat Polyclonal Antibody Against Mouse Il 36α, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+polyclonal+antibody+against+mouse+il+36%CE%B1/Mouse+IL-36+alpha%2FIL-1F6+Antibody/pmc12929778-330-8-23
    Average 93 stars, based on 21 article reviews
    goat polyclonal antibody against mouse il 36α - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Bone mineral loss damages renal tubules in mice"

    Article Title: Bone mineral loss damages renal tubules in mice

    Journal: Communications Biology

    doi: 10.1038/s42003-026-09603-0

    Total RNA and formalin-fixed paraffin sections were prepared from the kidneys from mice used in Fig. . Relative mRNA levels of renal tubular damage markers were measured by quantitative RT-PCR. a Osteopontin. b Neutrophil gelatinase-associated lipocalin (Ngal). c Kidney injury molecule-1 (Kim-1). d Interleukin-36α (IL-36α). e Interleukin-6 (IL-6). Data are indicated as violin plots with the median and quartiles (dotted lines). N = 6 for each column. * P < 0.05, # P < 0.01 versus baseline by one-way ANOVA with Tukey’s multiple comparison test. f Plasma creatinine levels. Data are presented as means ± SD. N = 6 for each column. No statistical differences were observed between the groups by one-way ANOVA with Tukey’s multiple comparison test. g Representative kidney section images of immunohistochemistry using antibodies against osteopontin (upper panels) or IL-36α (lower panels) at the indicated time points after the last sRANKL or vehicle injection. Bar = 1 mm.
    Figure Legend Snippet: Total RNA and formalin-fixed paraffin sections were prepared from the kidneys from mice used in Fig. . Relative mRNA levels of renal tubular damage markers were measured by quantitative RT-PCR. a Osteopontin. b Neutrophil gelatinase-associated lipocalin (Ngal). c Kidney injury molecule-1 (Kim-1). d Interleukin-36α (IL-36α). e Interleukin-6 (IL-6). Data are indicated as violin plots with the median and quartiles (dotted lines). N = 6 for each column. * P < 0.05, # P < 0.01 versus baseline by one-way ANOVA with Tukey’s multiple comparison test. f Plasma creatinine levels. Data are presented as means ± SD. N = 6 for each column. No statistical differences were observed between the groups by one-way ANOVA with Tukey’s multiple comparison test. g Representative kidney section images of immunohistochemistry using antibodies against osteopontin (upper panels) or IL-36α (lower panels) at the indicated time points after the last sRANKL or vehicle injection. Bar = 1 mm.

    Techniques Used: Quantitative RT-PCR, Comparison, Clinical Proteomics, Immunohistochemistry, Injection

    a The study design. Exogenous phosphate loading was induced by feeding a high phosphate diet (1.5% inorganic phosphate, “Pi”, HP diet) for 6 weeks, and endogenous phosphate loading was elicited by sRANKL administration using the triple dose regimen given every other week, respectively. b – f Relative mRNA levels of osteopontin ( OPN ), I L-36α, F4/80, Mcp-1 , and Collagen 1a1 quantified by qPCR. g Plasma FGF23 levels. Data are presented as means ± SD. N = 7–8 per group. Statistical significance was assessed by two-way ANOVA followed by Tukey’s multiple-comparison test; P valuses are indicated. Effect sizes of HP diet, sRANKL treatment, and their interaction were calculated from the ANOVA sums of squares and shown as partial η² (pη²).
    Figure Legend Snippet: a The study design. Exogenous phosphate loading was induced by feeding a high phosphate diet (1.5% inorganic phosphate, “Pi”, HP diet) for 6 weeks, and endogenous phosphate loading was elicited by sRANKL administration using the triple dose regimen given every other week, respectively. b – f Relative mRNA levels of osteopontin ( OPN ), I L-36α, F4/80, Mcp-1 , and Collagen 1a1 quantified by qPCR. g Plasma FGF23 levels. Data are presented as means ± SD. N = 7–8 per group. Statistical significance was assessed by two-way ANOVA followed by Tukey’s multiple-comparison test; P valuses are indicated. Effect sizes of HP diet, sRANKL treatment, and their interaction were calculated from the ANOVA sums of squares and shown as partial η² (pη²).

    Techniques Used: Clinical Proteomics, Comparison

    Plasma levels of phosphate ( a ), calcium ( b ), calcium phosphate product ( c ), CPPs ( d ), FGF23 ( e ), and TRAcP-5b ( f ) of the mice from the normal gravity group (1 G) and the microgravity group (0 G). Relative mRNA levels of osteopontin ( g ) and interleukin-36α (IL-36α) ( h ) in the kidney. Data are presented as means ± SD. N = 6 for each column. P values by t test are indicated.
    Figure Legend Snippet: Plasma levels of phosphate ( a ), calcium ( b ), calcium phosphate product ( c ), CPPs ( d ), FGF23 ( e ), and TRAcP-5b ( f ) of the mice from the normal gravity group (1 G) and the microgravity group (0 G). Relative mRNA levels of osteopontin ( g ) and interleukin-36α (IL-36α) ( h ) in the kidney. Data are presented as means ± SD. N = 6 for each column. P values by t test are indicated.

    Techniques Used: Clinical Proteomics

    Total RNA and formalin-fixed paraffin sections were prepared from the kidney samples from mice in Fig. . Relative mRNA levels of Osteopontin ( a ), Ngal , ( b ), Kim-1 ( c ), IL-36α ( d ), and matrix metalloprotease-3 ( MMP3 ) ( e ) were measured by quantitative RT-PCR. Data are indicated as violin plots with the median and quartiles (dotted lines). N = 7–12 for each column. P values by Kruskal–Wallis’s test with Dunn’s multiple-comparison test are indicated. f Representative kidney section images of hematoxylin-eosin staining (H&E) and immunohistochemistry using antibodies against osteopontin (OPN) or IL-36α from mice treated with sRANKL (upper panels) and mice co-treated with sRANKL and Risedronate (lower panels). Bar: 0.1 mm for H&E and 1 mm for immunohistochemistry.
    Figure Legend Snippet: Total RNA and formalin-fixed paraffin sections were prepared from the kidney samples from mice in Fig. . Relative mRNA levels of Osteopontin ( a ), Ngal , ( b ), Kim-1 ( c ), IL-36α ( d ), and matrix metalloprotease-3 ( MMP3 ) ( e ) were measured by quantitative RT-PCR. Data are indicated as violin plots with the median and quartiles (dotted lines). N = 7–12 for each column. P values by Kruskal–Wallis’s test with Dunn’s multiple-comparison test are indicated. f Representative kidney section images of hematoxylin-eosin staining (H&E) and immunohistochemistry using antibodies against osteopontin (OPN) or IL-36α from mice treated with sRANKL (upper panels) and mice co-treated with sRANKL and Risedronate (lower panels). Bar: 0.1 mm for H&E and 1 mm for immunohistochemistry.

    Techniques Used: Quantitative RT-PCR, Comparison, Staining, Immunohistochemistry

    Related Articles

    Blocking Assay:

    Article Title: Interleukin‐36α as a potential biomarker for renal tubular damage induced by dietary phosphate load
    Article Snippet: 2% (v/v) Triton X‐100, 1% BSA, and 5% rabbit serum (IL‐36a and Klotho) or 5% goat serum (F4/80), or a blocking reagent included in Histofine Simple Stain Mouse MAX PO (Nichirei Corporation, Tokyo, Japan) (Osteopontin). .. After blocking, the sections were incubated with a goat polyclonal antibody against mouse IL‐36α (diluted 1 : 100 with PBS containing 1% BSA; AF2297; R&D Systems, Minneapolis, MN, USA), a goat polyclonal antibody against mouse Klotho (diluted 1 : 100 with PBS containing 1% BSA; BAF1819; R&D Systems), a rat monoclonal antibody against mouse F4/80 (diluted 1 : 100 with PBS containing 1% BSA; GTX26640; GeneTex, San Antonio, TX, USA), or a mouse monoclonal antibody against mouse Osteopontin (diluted 1 : 100 with PBS containing 1% BSA; sc‐21742; Santa Cruz, Dallas, TX, USA) at 4 °C overnight and then with Histofine Simple Stain Mouse MAX PO (Nichirei Corporation) at room temperature for 1 h. Immunoreactivity was visualized by incubation with the DAB substrate kit (Dako; Agilent Pathology Solutions). ..

    Article Title: Bone mineral loss damages renal tubules in mice
    Article Snippet: The sections were blocked with phosphate buffered saline (PBS) containing 1% bovine serum albumin and 10% rabbit serum for IL-36α or a blocking reagent in Histofine Simple Stain Mouse MAX PO (Nichirei Corporation, Tokyo, Japan) for osteopontin. .. After blocking, the sections were incubated with a goat polyclonal antibody against mouse IL-36α (diluted 1: 100 with PBS containing 1% BSA; AF2297; R&D Systems, Minneapolis, MN, USA), a mouse monoclonal antibody against mouse osteopontin (diluted 1: 100 with PBS containing 1% BSA; sc-21742; Santa Cruz, Dallas, TX, USA), or rat monoclonal antibody against mouse F4/80 (diluted 1: 100 with PBS containing 1% BSA; GTX26640; GeneTex, San Antonio, TX, USA) at room temperature for 1 h and then with Histofine Simple Stain Mouse MAX PO (Nichirei Corporation) at room temperature for 30 min. Immunoreactivity was visualized by incubation with the DAB substrate kit (Dako; Agilent Pathology Solutions). ..

    Incubation:

    Article Title: Interleukin‐36α as a potential biomarker for renal tubular damage induced by dietary phosphate load
    Article Snippet: 2% (v/v) Triton X‐100, 1% BSA, and 5% rabbit serum (IL‐36a and Klotho) or 5% goat serum (F4/80), or a blocking reagent included in Histofine Simple Stain Mouse MAX PO (Nichirei Corporation, Tokyo, Japan) (Osteopontin). .. After blocking, the sections were incubated with a goat polyclonal antibody against mouse IL‐36α (diluted 1 : 100 with PBS containing 1% BSA; AF2297; R&D Systems, Minneapolis, MN, USA), a goat polyclonal antibody against mouse Klotho (diluted 1 : 100 with PBS containing 1% BSA; BAF1819; R&D Systems), a rat monoclonal antibody against mouse F4/80 (diluted 1 : 100 with PBS containing 1% BSA; GTX26640; GeneTex, San Antonio, TX, USA), or a mouse monoclonal antibody against mouse Osteopontin (diluted 1 : 100 with PBS containing 1% BSA; sc‐21742; Santa Cruz, Dallas, TX, USA) at 4 °C overnight and then with Histofine Simple Stain Mouse MAX PO (Nichirei Corporation) at room temperature for 1 h. Immunoreactivity was visualized by incubation with the DAB substrate kit (Dako; Agilent Pathology Solutions). ..

    Article Title: Bone mineral loss damages renal tubules in mice
    Article Snippet: The sections were blocked with phosphate buffered saline (PBS) containing 1% bovine serum albumin and 10% rabbit serum for IL-36α or a blocking reagent in Histofine Simple Stain Mouse MAX PO (Nichirei Corporation, Tokyo, Japan) for osteopontin. .. After blocking, the sections were incubated with a goat polyclonal antibody against mouse IL-36α (diluted 1: 100 with PBS containing 1% BSA; AF2297; R&D Systems, Minneapolis, MN, USA), a mouse monoclonal antibody against mouse osteopontin (diluted 1: 100 with PBS containing 1% BSA; sc-21742; Santa Cruz, Dallas, TX, USA), or rat monoclonal antibody against mouse F4/80 (diluted 1: 100 with PBS containing 1% BSA; GTX26640; GeneTex, San Antonio, TX, USA) at room temperature for 1 h and then with Histofine Simple Stain Mouse MAX PO (Nichirei Corporation) at room temperature for 30 min. Immunoreactivity was visualized by incubation with the DAB substrate kit (Dako; Agilent Pathology Solutions). ..

    Staining:

    Article Title: Interleukin‐36α as a potential biomarker for renal tubular damage induced by dietary phosphate load
    Article Snippet: 2% (v/v) Triton X‐100, 1% BSA, and 5% rabbit serum (IL‐36a and Klotho) or 5% goat serum (F4/80), or a blocking reagent included in Histofine Simple Stain Mouse MAX PO (Nichirei Corporation, Tokyo, Japan) (Osteopontin). .. After blocking, the sections were incubated with a goat polyclonal antibody against mouse IL‐36α (diluted 1 : 100 with PBS containing 1% BSA; AF2297; R&D Systems, Minneapolis, MN, USA), a goat polyclonal antibody against mouse Klotho (diluted 1 : 100 with PBS containing 1% BSA; BAF1819; R&D Systems), a rat monoclonal antibody against mouse F4/80 (diluted 1 : 100 with PBS containing 1% BSA; GTX26640; GeneTex, San Antonio, TX, USA), or a mouse monoclonal antibody against mouse Osteopontin (diluted 1 : 100 with PBS containing 1% BSA; sc‐21742; Santa Cruz, Dallas, TX, USA) at 4 °C overnight and then with Histofine Simple Stain Mouse MAX PO (Nichirei Corporation) at room temperature for 1 h. Immunoreactivity was visualized by incubation with the DAB substrate kit (Dako; Agilent Pathology Solutions). ..

    Article Title: Bone mineral loss damages renal tubules in mice
    Article Snippet: The sections were blocked with phosphate buffered saline (PBS) containing 1% bovine serum albumin and 10% rabbit serum for IL-36α or a blocking reagent in Histofine Simple Stain Mouse MAX PO (Nichirei Corporation, Tokyo, Japan) for osteopontin. .. After blocking, the sections were incubated with a goat polyclonal antibody against mouse IL-36α (diluted 1: 100 with PBS containing 1% BSA; AF2297; R&D Systems, Minneapolis, MN, USA), a mouse monoclonal antibody against mouse osteopontin (diluted 1: 100 with PBS containing 1% BSA; sc-21742; Santa Cruz, Dallas, TX, USA), or rat monoclonal antibody against mouse F4/80 (diluted 1: 100 with PBS containing 1% BSA; GTX26640; GeneTex, San Antonio, TX, USA) at room temperature for 1 h and then with Histofine Simple Stain Mouse MAX PO (Nichirei Corporation) at room temperature for 30 min. Immunoreactivity was visualized by incubation with the DAB substrate kit (Dako; Agilent Pathology Solutions). ..



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    goat polyclonal antibody against mouse il 36α  (R&D Systems)
    93
    R&D Systems goat polyclonal antibody against mouse il 36α
    Total RNA and formalin-fixed paraffin sections were prepared from the kidneys from mice used in Fig. . Relative mRNA levels of renal tubular damage markers were measured by quantitative RT-PCR. a Osteopontin. b Neutrophil gelatinase-associated lipocalin (Ngal). c Kidney injury molecule-1 (Kim-1). d <t>Interleukin-36α</t> (IL-36α). e Interleukin-6 (IL-6). Data are indicated as violin plots with the median and quartiles (dotted lines). N = 6 for each column. * P < 0.05, # P < 0.01 versus baseline by one-way ANOVA with Tukey’s multiple comparison test. f Plasma creatinine levels. Data are presented as means ± SD. N = 6 for each column. No statistical differences were observed between the groups by one-way ANOVA with Tukey’s multiple comparison test. g Representative kidney section images of immunohistochemistry using antibodies against osteopontin (upper panels) or IL-36α (lower panels) at the indicated time points after the last sRANKL or vehicle injection. Bar = 1 mm.
    Goat Polyclonal Antibody Against Mouse Il 36α, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+polyclonal+antibody+against+mouse+il+36%CE%B1/Mouse+IL-36+alpha%2FIL-1F6+Antibody/pmc12929778-330-8-23
    Average 93 stars, based on 1 article reviews
    goat polyclonal antibody against mouse il 36α - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Total RNA and formalin-fixed paraffin sections were prepared from the kidneys from mice used in Fig. . Relative mRNA levels of renal tubular damage markers were measured by quantitative RT-PCR. a Osteopontin. b Neutrophil gelatinase-associated lipocalin (Ngal). c Kidney injury molecule-1 (Kim-1). d Interleukin-36α (IL-36α). e Interleukin-6 (IL-6). Data are indicated as violin plots with the median and quartiles (dotted lines). N = 6 for each column. * P < 0.05, # P < 0.01 versus baseline by one-way ANOVA with Tukey’s multiple comparison test. f Plasma creatinine levels. Data are presented as means ± SD. N = 6 for each column. No statistical differences were observed between the groups by one-way ANOVA with Tukey’s multiple comparison test. g Representative kidney section images of immunohistochemistry using antibodies against osteopontin (upper panels) or IL-36α (lower panels) at the indicated time points after the last sRANKL or vehicle injection. Bar = 1 mm.

    Journal: Communications Biology

    Article Title: Bone mineral loss damages renal tubules in mice

    doi: 10.1038/s42003-026-09603-0

    Figure Lengend Snippet: Total RNA and formalin-fixed paraffin sections were prepared from the kidneys from mice used in Fig. . Relative mRNA levels of renal tubular damage markers were measured by quantitative RT-PCR. a Osteopontin. b Neutrophil gelatinase-associated lipocalin (Ngal). c Kidney injury molecule-1 (Kim-1). d Interleukin-36α (IL-36α). e Interleukin-6 (IL-6). Data are indicated as violin plots with the median and quartiles (dotted lines). N = 6 for each column. * P < 0.05, # P < 0.01 versus baseline by one-way ANOVA with Tukey’s multiple comparison test. f Plasma creatinine levels. Data are presented as means ± SD. N = 6 for each column. No statistical differences were observed between the groups by one-way ANOVA with Tukey’s multiple comparison test. g Representative kidney section images of immunohistochemistry using antibodies against osteopontin (upper panels) or IL-36α (lower panels) at the indicated time points after the last sRANKL or vehicle injection. Bar = 1 mm.

    Article Snippet: After blocking, the sections were incubated with a goat polyclonal antibody against mouse IL-36α (diluted 1: 100 with PBS containing 1% BSA; AF2297; R&D Systems, Minneapolis, MN, USA), a mouse monoclonal antibody against mouse osteopontin (diluted 1: 100 with PBS containing 1% BSA; sc-21742; Santa Cruz, Dallas, TX, USA), or rat monoclonal antibody against mouse F4/80 (diluted 1: 100 with PBS containing 1% BSA; GTX26640; GeneTex, San Antonio, TX, USA) at room temperature for 1 h and then with Histofine Simple Stain Mouse MAX PO (Nichirei Corporation) at room temperature for 30 min. Immunoreactivity was visualized by incubation with the DAB substrate kit (Dako; Agilent Pathology Solutions).

    Techniques: Quantitative RT-PCR, Comparison, Clinical Proteomics, Immunohistochemistry, Injection

    a The study design. Exogenous phosphate loading was induced by feeding a high phosphate diet (1.5% inorganic phosphate, “Pi”, HP diet) for 6 weeks, and endogenous phosphate loading was elicited by sRANKL administration using the triple dose regimen given every other week, respectively. b – f Relative mRNA levels of osteopontin ( OPN ), I L-36α, F4/80, Mcp-1 , and Collagen 1a1 quantified by qPCR. g Plasma FGF23 levels. Data are presented as means ± SD. N = 7–8 per group. Statistical significance was assessed by two-way ANOVA followed by Tukey’s multiple-comparison test; P valuses are indicated. Effect sizes of HP diet, sRANKL treatment, and their interaction were calculated from the ANOVA sums of squares and shown as partial η² (pη²).

    Journal: Communications Biology

    Article Title: Bone mineral loss damages renal tubules in mice

    doi: 10.1038/s42003-026-09603-0

    Figure Lengend Snippet: a The study design. Exogenous phosphate loading was induced by feeding a high phosphate diet (1.5% inorganic phosphate, “Pi”, HP diet) for 6 weeks, and endogenous phosphate loading was elicited by sRANKL administration using the triple dose regimen given every other week, respectively. b – f Relative mRNA levels of osteopontin ( OPN ), I L-36α, F4/80, Mcp-1 , and Collagen 1a1 quantified by qPCR. g Plasma FGF23 levels. Data are presented as means ± SD. N = 7–8 per group. Statistical significance was assessed by two-way ANOVA followed by Tukey’s multiple-comparison test; P valuses are indicated. Effect sizes of HP diet, sRANKL treatment, and their interaction were calculated from the ANOVA sums of squares and shown as partial η² (pη²).

    Article Snippet: After blocking, the sections were incubated with a goat polyclonal antibody against mouse IL-36α (diluted 1: 100 with PBS containing 1% BSA; AF2297; R&D Systems, Minneapolis, MN, USA), a mouse monoclonal antibody against mouse osteopontin (diluted 1: 100 with PBS containing 1% BSA; sc-21742; Santa Cruz, Dallas, TX, USA), or rat monoclonal antibody against mouse F4/80 (diluted 1: 100 with PBS containing 1% BSA; GTX26640; GeneTex, San Antonio, TX, USA) at room temperature for 1 h and then with Histofine Simple Stain Mouse MAX PO (Nichirei Corporation) at room temperature for 30 min. Immunoreactivity was visualized by incubation with the DAB substrate kit (Dako; Agilent Pathology Solutions).

    Techniques: Clinical Proteomics, Comparison

    Plasma levels of phosphate ( a ), calcium ( b ), calcium phosphate product ( c ), CPPs ( d ), FGF23 ( e ), and TRAcP-5b ( f ) of the mice from the normal gravity group (1 G) and the microgravity group (0 G). Relative mRNA levels of osteopontin ( g ) and interleukin-36α (IL-36α) ( h ) in the kidney. Data are presented as means ± SD. N = 6 for each column. P values by t test are indicated.

    Journal: Communications Biology

    Article Title: Bone mineral loss damages renal tubules in mice

    doi: 10.1038/s42003-026-09603-0

    Figure Lengend Snippet: Plasma levels of phosphate ( a ), calcium ( b ), calcium phosphate product ( c ), CPPs ( d ), FGF23 ( e ), and TRAcP-5b ( f ) of the mice from the normal gravity group (1 G) and the microgravity group (0 G). Relative mRNA levels of osteopontin ( g ) and interleukin-36α (IL-36α) ( h ) in the kidney. Data are presented as means ± SD. N = 6 for each column. P values by t test are indicated.

    Article Snippet: After blocking, the sections were incubated with a goat polyclonal antibody against mouse IL-36α (diluted 1: 100 with PBS containing 1% BSA; AF2297; R&D Systems, Minneapolis, MN, USA), a mouse monoclonal antibody against mouse osteopontin (diluted 1: 100 with PBS containing 1% BSA; sc-21742; Santa Cruz, Dallas, TX, USA), or rat monoclonal antibody against mouse F4/80 (diluted 1: 100 with PBS containing 1% BSA; GTX26640; GeneTex, San Antonio, TX, USA) at room temperature for 1 h and then with Histofine Simple Stain Mouse MAX PO (Nichirei Corporation) at room temperature for 30 min. Immunoreactivity was visualized by incubation with the DAB substrate kit (Dako; Agilent Pathology Solutions).

    Techniques: Clinical Proteomics

    Total RNA and formalin-fixed paraffin sections were prepared from the kidney samples from mice in Fig. . Relative mRNA levels of Osteopontin ( a ), Ngal , ( b ), Kim-1 ( c ), IL-36α ( d ), and matrix metalloprotease-3 ( MMP3 ) ( e ) were measured by quantitative RT-PCR. Data are indicated as violin plots with the median and quartiles (dotted lines). N = 7–12 for each column. P values by Kruskal–Wallis’s test with Dunn’s multiple-comparison test are indicated. f Representative kidney section images of hematoxylin-eosin staining (H&E) and immunohistochemistry using antibodies against osteopontin (OPN) or IL-36α from mice treated with sRANKL (upper panels) and mice co-treated with sRANKL and Risedronate (lower panels). Bar: 0.1 mm for H&E and 1 mm for immunohistochemistry.

    Journal: Communications Biology

    Article Title: Bone mineral loss damages renal tubules in mice

    doi: 10.1038/s42003-026-09603-0

    Figure Lengend Snippet: Total RNA and formalin-fixed paraffin sections were prepared from the kidney samples from mice in Fig. . Relative mRNA levels of Osteopontin ( a ), Ngal , ( b ), Kim-1 ( c ), IL-36α ( d ), and matrix metalloprotease-3 ( MMP3 ) ( e ) were measured by quantitative RT-PCR. Data are indicated as violin plots with the median and quartiles (dotted lines). N = 7–12 for each column. P values by Kruskal–Wallis’s test with Dunn’s multiple-comparison test are indicated. f Representative kidney section images of hematoxylin-eosin staining (H&E) and immunohistochemistry using antibodies against osteopontin (OPN) or IL-36α from mice treated with sRANKL (upper panels) and mice co-treated with sRANKL and Risedronate (lower panels). Bar: 0.1 mm for H&E and 1 mm for immunohistochemistry.

    Article Snippet: After blocking, the sections were incubated with a goat polyclonal antibody against mouse IL-36α (diluted 1: 100 with PBS containing 1% BSA; AF2297; R&D Systems, Minneapolis, MN, USA), a mouse monoclonal antibody against mouse osteopontin (diluted 1: 100 with PBS containing 1% BSA; sc-21742; Santa Cruz, Dallas, TX, USA), or rat monoclonal antibody against mouse F4/80 (diluted 1: 100 with PBS containing 1% BSA; GTX26640; GeneTex, San Antonio, TX, USA) at room temperature for 1 h and then with Histofine Simple Stain Mouse MAX PO (Nichirei Corporation) at room temperature for 30 min. Immunoreactivity was visualized by incubation with the DAB substrate kit (Dako; Agilent Pathology Solutions).

    Techniques: Quantitative RT-PCR, Comparison, Staining, Immunohistochemistry